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Epithelioma papulosum cyprini

WebWhen cell monolayers of epithelioma papulosum cyprini (EPC), fathead minnow (FHM), chinook salmon embryo (CHSE-214), and bluegill fry (BF2) were pretreated with 7% PEG-20,000, they produced 4-17-fold increases in plaque assay titers of IHNV. The plaque assay titers of viral hemorrhagic septicemia virus, chum salmon reovirus, and chinook salmon ... WebThis page lists the EVAg products (viruses) produced by using "Epithelioma papulosum cyprini (EPC)" for production cell line. The additional filters enable you with creating a sub-selection of items of this list. The search engine above will seek products into the complete catalogue. If you are interested in acquiring cell-lines, please visit ...

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WebMar 14, 2024 · Epithelioma Papulosum Cyprini (EPC) cells were maintained at 24°C in GMEM/HEPES 25 mM medium supplemented with 2 mM L-glutamine (PAA) and with 10% fetal bovine serum (FBS) (Eurobio) . rVHSV were propagated in monolayer cultures of EPC cells at 15°C as previously described . Virus titers were determined by plaque assays on … WebJan 1, 2010 · Initially established from proliferative skin lesions of the common carp, Cyprinus carpio L., the epithelioma papulosum cyprini (EPC) cell line (Fijan, … ethan outdoor furniture https://pamroy.com

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WebDexamethasone treatment decreases replication of viral hemorrhagic septicemia virus in Epithelioma papulosum cyprini cells [J]. Kim Min Sun, Lee Su Jin, Choi Seung Hyuk, Archives of virology . 2024,第5 期. 机译：地塞米松治疗降低了上皮细胞瘤中胞质瘤中的病毒出血性败血症病毒的复制 ... WebTransmission electron microscopy of epithelioma papulosum cyprini cells infected with spring viremia of carp virus (SVCV): (a) cell nucleus (N; scale bar = 1 μ m), showing depolymerization and... WebApr 1, 2024 · LMBV demonstrates strong infectivity in epithelioma papulosum cyprini (EPC) cells and can cause the cytopathic effect (CPE) in this cell line. EPC cells were cultured in Medium 199/EBSS (M199, HyClone) supplemented with 10% fetal bovine serum (FBS, HyClone) containing streptomycin (100 μg/mL) and penicillin (100 μg/mL) at 25 °C. ethan outlast 2

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Epithelioma papulosum cyprini

Current lineages of the epithelioma papulosum cyprini (EPC) cell …

WebOct 1, 2004 · Several methods to improve transfection of epithelioma papulosum cyprini (EPC) carp cells have been tested and are reported here. By modifying the cell cycle state of EPC cell monolayers and ... WebSep 15, 2024 · In this study, to know the role of HIF-1α in the apoptosis of fish cells, we produced HIF-1α knockout Epithelioma papulosum cyprini (EPC) cells using a …

Epithelioma papulosum cyprini

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WebEpithelioma papulosum cyprini cells were seeded at c. 90% confluency on a 48-well plate and incubated overnight in a 50-mg mL 1 IDU solution at 25 °C (Rovozzo & Burke 1973). Media was removed from the cell monolayers; the cells were then inoculated with tenfold dilutions of the virus sus-pension in IDU solution and incubated at room ... WebOct 28, 2024 · Epithelioma papulosum cyprini (EPC) cells were grown in Leibovitz medium (L-15, Sigma) enriched with 10% fetal bovine serum (FBS, Welgene) and penicillin-streptomycin (Welgene). Interferon regulatory factor 9 (IRF9) gene knockout EPC (ΔIRF9-EPC) cells established in our previous study [ 22] were also cultured under the same …

WebIn this study, to know the role of HIF-1α in the apoptosis of fish cells, we produced HIF-1α knockout Epithelioma papulosum cyprini (EPC) cells using a CRISPR/Cas9 vector, and a single cell clone showing a heterozygous insertion/deletion (indel) mutation (one nucleotide insertion and one nucleotide deletion in HIF-1α gene) was chosen for further … WebMar 1, 2012 · Epithelioma papulosum cyprini (EPC) cells were cultured in Leibovitz medium (L-15, Sigma) supplemented with penicillin (100 U/ml), streptomycin (100 μg/ml) and 10% fetal bovine serum (FBS, Gibco). The wild-type VHSV KJ2008 and the recombinant viruses rVHSV-wild and rVHSV-ΔNV-EGFP [18] were inoculated in …

WebVolume 33, Issue 8p. 701-704 Current lineages of the epithelioma papulosum cyprini(EPC) cell line are contaminated with fathead minnow, Pimephales promelas, … WebAbstract. MicroRNA-155 (miRNA-155) is known to play an important role in the regulation of innate and adaptive immune responses in mammals. However, no information is …

WebNov 21, 2016 · Epithelioma Papulosum Cyprini (EPC) Swine Influenza SIV: Madin - Darby Canine Kidney (MDCK) Swine Orthopox Virus - OPV: Porcine Kidney (PK-15), Porcine Primary Kidney (PPK) Viral Hemorrhagic Septicemia: Epithelioma Papulosum Cyprini (EPC), Fathead Minnow (FHM), Chinook Salmon Embryo (CHSE-214)

WebEPC [Epithelioma Papulosum Cyprini] is an epithelial cell line that was isolated in 1969 from the skin of a fathead minnow. This cell line was deposited by J Winton. Product category Animal cells Organism Pimephales promelas, Fathead minnow Cell type … ethan oversized sweatpantsethanox 323WebIn the present study, we found that the protein inhibitor of activated STAT 4a (PIAS4a) gene of fathead minnow (Pimephales promelas) was a target of miR-155, which was verified by the inhibitory activity of miR-155 in the expression of reporter gene harboring 3'UTR of PIAS4a of EPC cells. ethan outdoor furniture price listWeb西北农林科技大学水产科学系教授. 王高学，男， 汉族 ，1965年9月生，陕西省富平人，西北农林科技大学水产科学系教授，博士， 博士生导师 ，教育部水产类专业教学指导委员会委员；中国水产学会鱼病专业委员会委员；中国水产学会渔药专业委员会委员。. [1] ethanox 4701WebCurrent lineages of the epithelioma papulosum cyprini(EPC) cell line are contaminated with fathead minnow, Pimephales promelas, cells J Winton, J Winton US Geological Survey, Western Fisheries Research Center, Seattle, WA, USA Search for more papers by this author W Batts, W Batts ethan owusuWebJan 1, 2010 · Initially established from proliferative skin lesions of the common carp, Cyprinus carpio L., the epithelioma papulosum cyprini (EPC) cell line (Fijan, Sulimanovic, Bearzotti, Muzinic, Zwillenberg, Chilmonczyk, Vautherot & de Kinkelin 1983) has become one of the most widely used tools for research on fish viruses and the diagnosis of fish … ethanox 4706WebMar 22, 2024 · Furthermore, mutation of Leu27, Glu103, Lys117, and His165 markedly decreased the phosphorylation of signal transducer and activator of transcription (STAT) 1a induced by CiIFNa in the Epithelioma papulosum cyprini (EPC) cells, and Glu103 was shown to be required for the CiIFNa-activated antiviral activity. ethan overman hockey