WebMany eukaryotic transcription factors (TFs) contain intrinsically disordered low-complexity sequence domains (LCDs), but how these LCDs drive transactivation remains unclear. We used live-cell single-molecule imaging to reveal that TF LCDs form local WebJF549) Understanding Cas9 dynamics and its binding efficiency with chromatin [80] CASFISH (dCas9- ... RCasFISH SpdCas9 Halo ligands (Alexa Fluor 488, AF488) RNA imaging [105] CERTIS SpCas9 mScarlet-3×Flag lncRNA imaging, ... fluorescent protein (EGFP) Labeling of endogenous proteins [125] HITI NHEJ SpCas9 GFP Labeling of
Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and …
WebWe used cell-permeable JaneliaFluor549- or JaneliaFluor646-HaloTag ligands (JF549-Halo or JF646-Halo, respectively) to label the Halo-Na V 1.7, ... Briefly, hNa V 1.7R was tagged with an in-frame fusion of the fluorescent protein Venus … WebSep 1, 2024 · Janelia Fluor Shelf Life. Probes & Prep. organic-dyes. MJS88 July 31, 2024, 8:30pm 1. Hello all, I recently obtained some JF549-Halo ligand dye that I’d like to use in my experiments. The Promega protocol recommends diluting in DMSO, and cautions that this dilution is single-use. Given the high cost of the reagent, this seems slightly…insane. list of roofing materials for a shingle roof
Photobleaching profile of HaloTag-bound JF525, JF549 and …
WebWe also tested the Halo ligand JF549, which is a derivative of TMR with improved brightness and photostability [24]. We achieved excellent labelling of MukB-Halo-JF549 … WebPromega now offers select Janelia Fluor® HaloTag® Ligands in a new format to support an enhanced workflow with less waste. The lyophilized format provides the following benefits: Improved workflow through direct … WebIntroducing PrentaLux. Performance-based products and hospitality minded. Mixing these elegant shapes, sizes, colors, and textures creates the best combination of aesthetics … imitrex package insert