WebbpUC-PIA2K rApr, Kan, recombinant plasmid with partial IAH1 gene, and This study containing PGK1 P-ATF2-PGK1 T gene expression cassette Table 1. Strains and plasmids used in this study. The plasmid pUC-PIA2K was constructed by insertion of the ATF2 ORF into the BglII restriction site in the yeast phosphoglycerate kinase I gene PGK1. The … Webb1 sep. 2012 · To date, five distinct proteins — Atf1p, Atf2p, Eht1p, Eeb1p and Iah1p — have been identified and characterized in S. cerevisiae as having ester synthesis or hydrolysis activity, with the alcohol acetyltransferase Atf1p having the greatest activity and being the most studied (Lilly et al. 2000, 2006a; Saerens et al. 2010; reviewed by …
Acetate-ester hydrolase activity for screening of the variation in ...
WebbGene summary Gene standard name iah1 Systematic ID SPCC126.10 Product isoamyl acetate hydrolytic enzyme Iah1 Organism Schizosaccharomyces pombe (fission yeast) … Webbisoamyl acetate esterase; IAH1 (gene name) Class Hydrolases; Acting on ester bonds; Carboxylic-ester hydrolases BRITE hierarchy Sysname 3-methylbutyl acetate acetohydrolase Reaction (IUBMB) 3-methylbutyl acetate + H2O = 3-methylbutanol + acetate [RN: R12516] Reaction (KEGG) R12516 Reaction Substrate 3-methylbutyl … fish restaurants in lymington
iah1 putative isoamyl acetate hydrolytic enzyme Iah1 [ (fission yeast)]
Webb27 sep. 2024 · To conclude, in this study single and double gene knockouts of IAH1 and TIP1 were constructed using CRISPR-Cas (i.e., ΔIAH1, ΔTIP1 and ΔIAH1ΔTIP1). … WebbSAN TA C RUZ BI OTEC HNOL OG Y, INC . IAH1 (C-12): sc-137523 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax831.457.3801 Europe +0080045738000 ... WebbWe constructed the IAH1 gene expression system in Escherichia coli, and purified the IAH1 gene product (Iah1p). The amount of Iah1p produced by recombinant E. coli was more than 40% of total cellular proteins. The molecular size of Iah1p was 28 kDa by SDS-polyacrylamide gel electrophoresis. fish restaurants in malaga